الرئيسية / علماء مصر (الطيور المهاجرة) / Tarek Dishisha , *; Adel Abouhamed ; Gashaw Mamo ; Magdy Aly Amin ; Rajni Hatti-Kaul (AEA-Scholars)

Tarek Dishisha , *; Adel Abouhamed ; Gashaw Mamo ; Magdy Aly Amin ; Rajni Hatti-Kaul (AEA-Scholars)

Tarek Dishisha , *; Adel Abouhamed ; Gashaw Mamo ; Magdy Aly Amin ; Rajni Hatti-Kaul

Association of Egyptian-American Scholars

Background:

Multi-drug resistant microorganisms are creating a serious problem for infection control in Egyptian hospitals. Several innovation alternatives to the conventional antibiotics have been proposed including the use of antimicrobial peptides, bacteriocins, cell lytic enzymes, and bacteriophages. Phage-derived lysins (endolysins) are a new class of antimicrobials acting by hydrolysing the peptidoglycan layer of bacterial cell wall.

Purpose:

Development of innovative formulations for facile application of phage-derived lysins in prophylaxis and treatment of microbial infections.

Methods:

Bacteriophage T4 lysozyme (T4Lyz); a product of gene e; was fused with Cellulose Binding Module (CBM) from Cellulomonas fimi. Genes encoding T4Lyz and T4Lyz-CBM were cloned and expressed in Escherichia coli. The resulting enzymes were purified and evaluated with regards to optimum pH, stability, lytic activity and bactericidal activity. The recombinant enzymes were immobilised on cellulosic wound dressing gauze as well as chitosan nanoparticles.

Results:

The CBM tag did not alter the optimum pH and stability of T4Lyz, while the lytic activity was reduced. The bactericidal activity of T4Lyz-CBM was 97.5% against Micrococcus lysodeikticus (using 10 µg/mI protein solution), and 99.96%and 95% against E.coli and Pseudomonas mendocina, respectively, (using 200 µg/mI protein solution). In contrast to unmodified T4Lyz, T4Lyz-CBM was successfully and irreversibly immobilised to cellulosic dressing gauze. The immobilised fusion protein retained its bactericidal activity against both Gram- positive and –negative bacteria. Immobilisation also enhanced the enzyme compared to that of the free one. The recombinant proteins were also immobilised over chitosan and cellulose nanomaterials however the activity was significantly reduced.

Conclusions:

Phage-derived lysins is a potential alternative to the conventional antibiotics. Proper engineering of these lysins would facilitate their formulations for medical applications. Tagging T4Lyz with CBM provides a facile, irreversible binding to cellulosic wound dressing while retaining its activity. Currently, the development of lysins against several notorious multi-drug resistant microorganisms that are widely spread in the Egyptian community is being conducted.

This article was published in 3alamaltanmya

sponsored by Future Builders International Academy

Led by Dr.Maha Fouad

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شاهد أيضاً

(Mohab S. El-Moiazen, Laila A. Mosaad (AEA-Scholars

Mohab S. El-Moiazen, Laila A. Mosaad Association of Egyptian-American Scholars Background: World Health Organization proved …

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  293. Zune and iPod: Utmost Those people compare the Zune towards the Touch, yet when seeing how slim and surprisingly very low and light-weight it is, I acquire it in direction of be a pretty special hybrid that brings together attributes of both the Touch and the Nano. It is Pretty colourful and lovely OLED display screen is marginally lesser than the contact screen, nonetheless the participant alone feels Extremely a little bit lesser and lighter. It weighs above 2/3 as significantly, and is significantly smaller inside of width and top, despite the fact that being accurately a hair thicker.

  294. This is having a little bit added subjective, yet I much prefer the Zune Current market. The interface is colourful, contains extra aptitude, and some awesome capabilities like ‘Mixview’ that enable oneself instantly see identical albums, music, or other end users very similar towards what you happen to be listening in the direction of. Clicking on 1 of all those will centre on that products, and a different set of “neighbors” will come into perspective, allowing for you toward navigate more than exploring through related artists, tunes, or customers. Talking of end users, the Zune “Social” is moreover Good enjoyable, enabling oneself obtain some others with shared tastes and turning into pals with them. By yourself then can pay attention toward a playlist built centered upon an amalgamation of what all your pals are listening towards, which is much too fascinating. All those apprehensive with privacy will be relieved towards notice you can protect against the public from observing your person listening behaviors if yourself thus pick.

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  296. Concerning me and my spouse we have owned more MP3 avid gamers around the decades than I can count, together with Sansas, iRivers, iPods (classic & touch), the Ibiza Rhapsody, and so on. But, the previous handful of a long time I’ve fixed down to one line of players. Why? Since I was content in direction of explore how well-designed and exciting in the direction of employ the underappreciated (and extensively mocked) Zunes are.

  297. If you’re nonetheless upon the fence: get your favorite earphones, intellect down in the direction of a Easiest Buy and request in direction of plug them into a Zune then an iPod and see which a person appears better in the direction of by yourself, and which interface tends to make you smile excess. Then you can expect to recognize which is specifically for oneself.

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  309. The fresh new Zune browser is remarkably very good, still not as Wonderful as the iPod’s. It works nicely, still isn’t really as fast as Safari, and is made up of a clunkier interface. If your self often application upon having the world wide web browser that is not an issue, still if you might be planning toward examine the web alot towards your PMP then the iPod’s greater exhibit and superior browser may possibly be significant.

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  311. Apple at present is made up of Rhapsody as an application, which is a excellent get started, nevertheless it is presently hampered by way of the incapability toward store locally on your iPod, and is made up of a dismal 64kbps little bit rate. If this alterations, then it will somewhat negate this advantage for the Zune, but the Ten tunes for every thirty day period will still be a substantial plus within just Zune Pass’ like.

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